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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۲۹-۳۰
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عنوان انگلیسی Ps-21: Characterization and Comparing Amniotic Fluid Stem Cells before and after Vitrification
چکیده انگلیسی مقاله Objective: Recent researches have indicated that amniotic fluid stem cells can provide unique source for clinical applications and regenerative medicine. The aim of this study was to compare amniotic fluid derived stem cells (AFSCs) before and after vitrification/warming in optimized culture condition for efficient long-term storage for therapeutic aims. Materials and Methods: The amniotic fluid samples were obtained with informed consent of 6 pregnant women during an amniocentesis procedure in Teaching- Alzahra hospital of Tabriz, Iran. Samples were centrifuged at 1200 rpm for 10 min to obtain a pellet. The cells were seeded in 6 well plates without feeder layers and cultured in optimized culture condition separately for 4th passages, then isolated stem cells were vitrified/ warmed and compared for gene expression and specific CD markers in both fresh and vitrified conditions using RT-PCR and flowcytometry, repectively. Results: The present study demonstrated that both fresh and vitrified AFSCs had high growth rates with stem cell-like cell morphology in optimized culture condition and they showed adherent properties in plastic surface. Fresh/vitrified AFSCs showed positive expression of Oct4 and Nanog genes and negative expression of CD31and CD45 markers which were equal in both fresh and vitrified cells. In contrast, expression of CD44 and CD90 in fresh cells were more than vitrified cells. Conclusion: Our findings indicated that vitrification/ warming did not have any negative effects on specific markers and gene expression of AFSCs. We will further analyze our stem cells for differentiation abilities. Our data showed promising result for efficient long-term storage of AFDS cells for therapeutic aims in the future.
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نویسندگان مقاله m پاشایی اصل | m pashai asl


z fardyazar | z fardyazar


e فتحی | e fathi


r فرحزادی | r farahzadi


f پاشایی اصل | f pashaiasl


m رحمتی یامچی | m rahmati yamchi


sh قلیزاده قلعه عزیز | sh gholizadeh ghaleh aziz


نشانی اینترنتی http://celljournal.org/journal/article/abstract/77
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