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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۰-۳۰
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عنوان انگلیسی Ps-22: Migration of Bone Marrow-Derived Very Small Embryonic-Like Stem Cells Toward The Injured Spinal Cord
چکیده انگلیسی مقاله Objective: Different ways have been tested for stem cells delivery in cell therapy experiments. One of the most convenient ways of stem cells administration is intravenous infusion. Very small embryonic-like stem cells (VSELS) are a rare population of stem cells in bone marrow that show some embryonic and adult stem cell characters. Ischemic was associated with significant increase on the number of circulating VSELS in PB. we transplanted very small embryonic like stem cells by intravenous injection in order to clarify whether these transplanted cells migrate into the injured spinal cord lesion. Materials and Methods: Mouse BM aspirated from femur and then VSELS were isolated with FACS and cultured on C2C12 feeder layer cells. After characterization with immunocytochemistry, labeled VSELS injected via tail vein to compression spinal cord injury model rats. 4 weeks after transplantation, tissue was assessed with fluorescence microscopy and homming was evaluated by real-time PCR analysis and histological study for identification of injected population in site of injury Results: Findings show that Very small embryonic-like stem cells can be isolated from BM by FACS and expanded in vitro, revealed a large number of DiI-labelled cells in the lesion. Real-time PCR analysis confirmed that the expression of Oct4 mRNA was significantly increased in the injury site. SSEA1 antibody positive also demonstrated that VSELS had migrated into the lesion. Conclusion: Very small embryonic-like stem cells of VSELS into PB occurs in rat with spinal cord injury.identified in mice and the mobilization
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نویسندگان مقاله z گلی پور چوشلی | z golipoor choshali


f مهرآیین | f mehr aein


m باعزم | m baazm


f ظفری | f zafari


a علیزاده | a alizadeh


sh آب آب زاده | sh ababzadeh


نشانی اینترنتی http://celljournal.org/journal/article/abstract/78
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