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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۰-۳۰
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عنوان انگلیسی Ps-23: The Influence of Cerebrospinal Fluid Accompanied by Retinoic Acid on Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells In Vitro
چکیده انگلیسی مقاله Objective: Cerebrospinal fluid (CSF) has a broad range of molecules, growth and neurotrophic factors which are essential for neurogenesis in embryos and adults. Bone marrow mesenchymal stem cells (BMSCs) are multipotent stem cells that can differentiate into the cells with neural-like phenotype under the induction of appropriate growth factors. According to the significant role of retinoic acid (RA) in neurogenesis, the aim of this study was to induce differentiation of BMSCs into neuron-like cells using CSF, RA and combinative form of CSF and RA. Materials and Methods: Bone marrow mesenchymal stem cells were isolated and characterized. The CSF was prepared from cisterna magna of 19 days Wistar rat embryos. Then BMSCs were induced by 5% CSF (CSF group), 10-6 �M retinoic acid (RA group) and CSF plus RA (CSR group) for 12 days. Morphology of differentiated cells was examined by inverted microscope and axonal outgrowth was measured using the image J software. In addition, the expression of neuralspecific markers (Nestin and MAP-2) was examined by Immunocytochemistry. Results: The specific - neuronal morphology was observed in differentiated cells. The maximum axon length was seen in CSR group on 12th day of induction. The results of immunocytochemistry showed the expression of Nestin, a neuroprogenitor marker, in all treated groups. However, MAP-2, known as a mature neural marker, was expressed only in CSR group. Conclusion: According to the findings of this study, the synergistic effect of CSF and RA enhances the differentiation of BMSCs into neuronal and glial phenotypes in vitro.co
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نویسندگان مقاله g گودرزی | g goudarzi


hg حمیدآبادی | hg hamidabadi


a خلعتبری | a khalatbari


a عبدانی پور | a abdanipour


hm کوچصفهانی | hm kouchesfehani


aen مقدم | aen moghaddam


نشانی اینترنتی http://celljournal.org/journal/article/abstract/79
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