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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۱-۳۱
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عنوان انگلیسی Ps-24: The Effect of Cyclic Uniaxial Stretch on Tenogenic Differentiation of Rabbit Adipose-Derived Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Tissue engineering using stem cells is a promising method for tendon regeneration. Cyclic stretch is one of the important factors affecting differentiation of mesenchymal stem cells (MSCs) into tenocytes. Utilization of such mechanical signal may improve tenogenic differentiation of stem cells in vitro and can be used in tendon tissue engineering applications. Materials and Methods: The aim of this work was to study the effect of cyclic strain on tenogenic differentiation of rabbit adipose-derived mesenchymal stem cells (rADSCs). After isolation of rADSCs from abdominal fat tissue, characterization by flow cytometry and assessment of multilineage differentiation potential, pellets of passage 3 cells were cultivated on a PolyDi- MethylSiloxane (PDMS) substrate. A cyclic uniaxial stretch device, which had been previously developed in National Cell Bank of Iran, Pasteur Institute of Iran, was used for mechanical stimulation.The substrate was subjected to cyclic stretch (10% strain, 1 Hz frequency) for 24 h. Real-time PCR method was used to examine the expression of collagen type I (col I) and decorin as two important tenogenic markers. Results: Regarding flow cytometry results, expression levels of cell surface proteins were highly characteristic of MSCs. Multipotency of isolated rADSCs was confirmed by their multilineage differentiation. According to real-time PCR results, application of cyclic uniaxial stretch increased the expression of Col I (by 6 folds) and Decorin (by 1.5 folds) compared to the control group. Conclusion: The higher expression of Col I and Decorin, in response to mechanical stretch, indicates the positive effect of such mechanical signal on tenogenesis and that uniaxial stretch can be used as an effective tool in rADSC stenogenic differentiation and tendon tissue engineering.
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نویسندگان مقاله m hamrangsekachaee | m hamrangsekachaee


n حقیقی پور | n haghighipour


s بنکدار | s bonakdar


نشانی اینترنتی http://celljournal.org/journal/article/abstract/80
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