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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۱-۳۱
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عنوان انگلیسی Ps-25: Investigation of von Willebrand Factor (vWF) Gene Expression in Endothelial Cells Differentiated from Adipose-Drived Mesenchymal Stem Cells and its Stability
چکیده انگلیسی مقاله Objective: Vascular endothelial cells are subjected to shear and tensile stresses induced by blood flow and pressure, respectively. Cells assigned to cover the tissue engineered vessels have been already isolated from several human sources such as blood vessels, bone marrow, and recently adipose tissue. There have been studies concerning the differentiation of adult stem cells into ECs, mostly using mesenchymal stem cells (MSCs). When subjected to different mechanical stimulations, MSC differentiation varies. For example, Fluid shear stress can induce their differentiation towards endothelial cells and the process is believed to play an important role in vasculogenesis and cardiac repair. Materials and Methods: Human adipose-drived mesenchymal stem cells (AdMSCs), were characterized in passage 3 using flow cytometry. In order to mimic the in vivo conditions, AdMSCs were cultured in tubular silicone scaffolds, and then exposed to endothelial growth factor (VEGE) for 7 days or shear stress of 4/49 dyn/cm2 for 24 hours, using a perfusion bioreactor. In order to examine the differentiation, the expression level of vWF, as an endothelial specific gene, was studied using real-time PCR, and for studying the stability of differentiation, the mRNA levels of this gene were quantified, upon completion of each test, as well as on the 5th and 10th days after the test. Results: According to real-time PCR results, immediately upon the completion of mechanical and chemical tests as well as on the 5th and 10th days after the experiments, the mRNA level of vWF was higher in the mechanical group (approximately 5.28, 1.18 and 2.97 respectively), compared to the chemical ones (approximately 1.1, 1.69 and 1.28 respectively). Conclusion: The findings of this study indicate that shear stress alone can be an endothelial differentiation factor by itself. It was demonstrated that in 10 days after exposure of mesenchymal stem cells to shear stress and chemical signals, stability in the expression of vWF gene could be observed. The results of this study can be used as a tool to achieve a practical and stable endothelial differentiation of ADSCs, for application in cardiovascular treatments.
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نویسندگان مقاله e حسن زاده | e hasanzadeh


gh عموعابدینی | gh amoabediny


n حقیقی پور | n haghighipour


n غلامی | n gholami


n امیرزاده | n amirzadeh


j محمدنژاد | j mohammadnejad


نشانی اینترنتی http://celljournal.org/journal/article/abstract/81
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