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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۲-۳۲
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عنوان انگلیسی Ps-27: Estrogen Receptor Beta: A Novel Transcription Factor for Aromatase Coding Gene (CYP19A1) Regulation in Cumulus Cells
چکیده انگلیسی مقاله Objective: The pivotal functions of aromatase and its product intrafollicular estrogen in regulation of follicular growth and development are well known. Estrogen mainly mediates its effects in ovarian cells through estrogen receptor β (ERβ). ERβ can act in both up- and downregulation of target genes via recognizing binding sites on genome. Despite several reports focused on aromatase, the regulation of aromatase coding gene (CYP19A1) expression has not been extensively studied in human cumulus cells (CCs). CYP19A1 is expressed in ovarian cells via an ovarian-specific promoter II (PII). Furthermore, given the ability of ERβ to binds to ERE half sites (estrogen responsive elements half sites) that exist in PI.3 and PI.4, we challenged the hypothesis that the ERβ may be a regulator of aromatase expression by investigate incorporation of ERβ on promoter regions of CYP19A1 gene. Materials and Methods: Cumulus oocyte complexes were obtained from 20 infertile patients with tubal factors or/and egg donor who underwent ovarian stimulation with GnRH agonist or antagonist for intracytoplasmic sperm injection. Only the CCs from MII oocytes were selected for this study. The association of ERβ with three promoter regions (PII, PI.3 and PI.4) of CYP19A1 gene was examined using Chromatin Immunoprecipitation (ChIP) assay. Results: Our data revealed that ERβ are incorporated into the three analyzed regulatory regions; however, in women with GnRH agonist protocol, its incorporation on promoter PII is significantly higher than other promoters. In contrast, in women with GnRH antagonist protocol, incorporation of ERβ on promoter PI.3 and PI.4 is significantly higher than promoter PII. Conclusion: Our results have shown that human cumulus cell aromatase is controlled, in part, by locally produced estrogen hormone through its receptor (i.e., ERβ). This steroid hormone receptor can be added to the other regulatory transcription factors which direct the expression of the CYP19A1 gene in human cumulus cells.
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نویسندگان مقاله e حسینی | e hosseini


f مهرآیین | f mehraein


l کریمیان | l karimian


p افشاریان | p afsharian


m p اشرفی | m p ashrafi


m شاه حسینی | m shahhoseini


نشانی اینترنتی http://celljournal.org/journal/article/abstract/83
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