این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۴-۳۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-32: miRNAs Expression Profile during Osteogenic Differentiation of Human Adipose-Derived Stem Cells Cultured on Aligned Biodegradable Electrospun Nanofibrous Scaffold
چکیده انگلیسی مقاله Objective: Bone tissue engineering is an interesting approach for the treatment of Bone fractures, osteoporosis, malformation, and clinically important bone diseases. Electrospun poly-L-lactide (PLLA) aligned nanofibers improve osteogenic differentiation of mesenchymal stem cells because of their niche-mimicking features exploited to regulate stem cell attachment, proliferation, differentiation and function in vitro and in vivo. Osteoblast differentiation is tightly regulated by post transcriptional regulators such as microRNAs; thus controlling miRNA activity in hMSCs can be an effective tool for enhancing the induction of osteogenesis for tissue engineering purposes. Materials and Methods: In this study, aligned PLLA nanofibrus scaffolds were fabricated using electrospinning technique. Human adipose tissue derived stem cells (hAD-MSCs) were seeded on aligned electrospun fibrous with an initial cell density of 2 × 105 cells per cm2 and incubated in the osteogenic medium for 21 days. Then we investigated the miRNA expression profile during osteogenic differentiation of hADSCs, and assessed the role of nanofibrous topographies on the osteogenic differentiation potential of hADSCs in comparison with TCPS. Putative targets of the miRNAs were predicted using online software miRanda, TargetScan, miRBase which identified its regulatory network, molecular function, biological processes and its target genes involved in negative regulation of osteoblast differentiation. miRNA expression profiles during osteogenic differentiation of MSCs were verified using quantitative real-time polymerase chain reaction (qRT-PCR). Furthermore,TGF-beta signaling pathway activity has been analyzed as an important regulatory pathway of osteogenesis. After 7, 14, 21 days osteogenic commitment of osteoblasts was evaluated by measuring ALPase activity, extracellular calcium deposition ,specific staining and evaluation of some main osteogenic marker genes. Results: These findings demonstrate that hADSCs, cultured on electrospun PLLA aligned fibers, had higher ALP activity and calcium deposition in comparison with Tcps. Thus aligned nanofibrus provides a good environment for cell growth and osteogenic differentiation and have great potential in the bone tissue engineering. Furthermore, our study reveals more details about osteogenic differentiation of hADSCs and suggests controlling miRNA activity in hMSCs, as a novel biomarker, through effect of nanofibrus scaffolds on miRNAs profiling and pathways involved in osteogenesis. Taking together, it can be an effective tool for enhancing the induction of osteogenesis for tissue engineering purposes. Conclusion: These results suggest that PLLA aligned nanofibers could influence on the expression of miRNAs, as a novel approach for controlling osteoblast differentiation.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله m ایزدپناهی | m izadpanahi


e سیدجعفری | e seyedjafari


e عارفیان | e arefian


m سلیمانی | m soleimani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/88
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات