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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۵-۳۵
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عنوان انگلیسی Ps-33: Characterization of Neural Stem Cells Isolated from Adult Rhesus Macaque Monkey’s Subventricular Zone
چکیده انگلیسی مقاله Objective: Neural stem cells of adult mammalian brain have the potential ability to be used as a source of tissue of autologous cell replacement strategies for the cell treatment of neurodegenerative diseases. Neural stem cells (NSCs) are self-renewable in culture and can generate mature neural progeny which display the characteristics of functional neurons and glia. This cell population could therefore be suitable source for cell therapy in the treatment of neurodegenerative disease. Our goal in this study was to characterize the neural stem cells isolated from subventricular zone. Materials and Methods: The subventricular zone (SVZ) was microdissected from Rhesus Macaque monkey (male) and dissociated with trypsin, the single cell was cultured into NSC medium. Flow cytometry analysis, Immunofleoroscent staining and qRT-PCR were performed in the fourth passage. Karyotype of isolated neural stem cells from Monkey’s SVZ was also checked. Furthermore, the ability of colony formation from single cell was evaluated. Results: Immunofleoroscent staining and flow cytometry analysis of NSC-SVZ showed that these cells were NESTIN+, GFAP+, SOX1+, SOX2+ and PAX6+ in the expansion of Neural stem cell medium. NESTIN, GFAP, SOX1, SOX2 and PAX6 showed high level of gene expression at the forth passage. Karyotype analysis showed a normal male chromosome complement (42, XY). Conclusion: Our study demonstrated that the cells have characteristics of neural stem cells. It seems that these cells were suitable option for cell replacement of neurodegenerative diseases.
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نویسندگان مقاله r جابری | r jaberi


sh نعمتی | sh nemati


r جباری | r jabari


h بهاروند | h baharvand


s کیانی | s kiani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/89
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