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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۵-۳۶
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عنوان انگلیسی Ps-34: Evaluation of SOX9 Expression in 3D-Cultured Stem Cells Using A Specialized Cartilage Tissue Engineering Bioreactor
چکیده انگلیسی مقاله Objective: Articular cartilage is an avascular tissue with limited intrinsic healing capacity. Among all possible cell sources for cartilage repair, mesenchymal stem cells (MSCs) have attracted a great deal of interest for such application. Matrix synthesis enhancement for cell-based cartilage regeneration aims at providing anchorage support to cells and localizing them in the injury site after implantation in order to prevent delamination and failure of the graft. It is becoming increasingly clear that differentiation of MSCs is controlled by mechanical signals. Mechanical forces which are generated within the cell in response to its surrounding extracellular matrix play major roles in determining cell fate. The aim of this study is to assess the effects of cyclic hydrostatic pressure on chondrogenesis of encapsulated MSCs. Materials and Methods: Rabbit adipose-derived (AD)-MSCs and chondrocytes were obtained from National Cell Bank of Iran (Pasteur Institute of Iran) and at passage 4, they were encapsulated in alginate hydrogels and then incubated at 37°C for 0, 7 and 14 days. Thereafter, half of the samples were exposed to 5 MPa of cyclic hydrostatic pressure at 0.5 Hz for 7 days and the remaining samples were incubated at 37oC and used as the control group. Cellular toxicity, adhesion and proliferation of encapsulated cells were evaluated. The expression of SOX9 in encapsulated cells after hydrostatic pressure application was quantified using realtime PCR. Results: According to cell adhesion and proliferation results, alginate capsules which were used for 3D cell culture resulted in higher cell densities and provided better microenvironments for cells, compared to 2D culture plates. Based on the MTT assay results, all cell viabilities in alginate capsules after 3, 7 and 14 days were greater than 90%. The expression of Sox9, as an important chondrogenic marker, in MSCs was increased by 3.5 folds in response to the application of hydrostatic pressure, compared tothe control group. The expression of this gene was increased over time, so that it was 2 times higher on day 21 compared to that on day 7. Conclusion: According to proliferation and MTT assays, alginate provides a good 3D environment for cells. The application of hydrostatic pressure on alginate-encapsulated MSCs increases the expression of Sox9, as a chondrogenic gene, and can be used in cartilage tissue engineering applications. Such alginate structure with differentiated MSCs can be injected to cartilage defects.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله a جهانبخش | a jahanbakhsh


ms نوربخش | ms nourbakhsh


ma شکرگزار | ma shokrgozar


n حقیقی پور | n haghighipour


s بنکدار | s bonakdar


نشانی اینترنتی http://celljournal.org/journal/article/abstract/90
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