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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۶-۳۷
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عنوان انگلیسی Ps-36: Effect of 1α, 25-Dihydroxy Vitamin D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous Teeth
چکیده انگلیسی مقاله Objective: Stem cells from human exfoliated deciduous teeth (SHEDs) are a population of highly proliferative cells capable of differentiating into osteogenic, odontogenic, adipocytes and neural cells. Vitamin D3 metabolites such as 1α,25-dihydroxy vitamin D3 are key factors in the regulation of bone metabolism. The aim of this study was to investigate the effect of 1α,25- dihydroxy vitamin D3 on osteogenic differentiation (alkaline phosphatase activity and Alizarin red staining) of stem cells of exfoliated deciduous teeth. Materials and Methods: Dental pulp was removed from freshly extracted primary teeth and immersed in a digestive solution. Then dental pulp cells were immersed in α-MEM culture medium, added with 10% FBS (fetal bovine serum). After the third passage, cells were isolated from the culture plate and were used for osteogenic differentiation. As a control group, cells were cultured in osteogenic cell culture medium and as a case group cells were cultured in osteogenic culture medium supplemented with 100 nM 1α,25(OH)2D3. Alkaline phosphatase (ALP) activity and alizarin red staining were analyzed to evaluate the osteogenic differentiation at day 21. The results were analyzed using t-test. Results: Significant increase was in ALP activity of SHEDs after treatment with 1α,25(OH)2D3 in comparison to cells in control group (P=0.002). Alizarin red staining demonstrated that cells exposed to 1α,25(OH)2D3 induced higher mineralized nodules (P< 0.001). Conclusion: Osteoblast differentiation in SHEDs was stimulated by 1α,25(OH)2D3. Based on these findings, we conclude that 1α,25 (OH) 2D3 can be used as osteogenic inducer in tissue engineering.
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نویسندگان مقاله a جانشین | a janeshin


m مرتضوی | m mortazavi


f مجرد | f mojarad


i امیری | i amiri


نشانی اینترنتی http://celljournal.org/journal/article/abstract/92
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