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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۷-۳۸
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عنوان انگلیسی Ps-39: Hypoxic Preconditioning Protects Bone Marrow Mesenchymal Stem Cells against Induced Apoptosis and Enhances Cell Proliferation and Viability
چکیده انگلیسی مقاله Objective: Poor cell survival ability and limited functional benefits have restricted the efficacy of bone marrow mesenchymal stem cells (BMMSCs) in cell therapy. Recently, it has been reported that hypoxia preconditioning of BMMSCs in vitro before transplantation can enhance the survival and therapeutic properties of these cells. Present study investigated the effect hypoxic preconditioning of mesenchymal stem cells with hydrogen peroxide with (H2O2) and cobalt chloride (CoCl2) on cells proliferation, viability and apoptosis in vitro. Materials and Methods: Bone marrow mesenchymal stem cells preconditioned with H2O2, CoCl2 as hypoxia mimetic agent and both H2O2, CoCl2 for 6, 12, 24 and48 hours. Then, the MSCs were exposed to lethal dose of H2O2 (300 μM) for 24 hours followed by 24 hours recovery. MTT assay and trypan blue staining were conducted to evaluate the cell proliferation and viability. Also, TUNEL assay was done to study the cell apoptosis. Results: According to our data, cell proliferation in group preconditioned groups increased significantly compared to the other groups after 6, 12, 24 and 48 hours (P< 0.05). Cell survival significantly increased in preconditioned groups with H2O2 and hypoxia after 6, 12, 24 and 48 hours (P< 0.05). Moreover, cell apoptosis was decreased in preconditioned groups significantly than control group after 6, 12, 24 and 48 hours (P< 0.05). Conclusion: This study clearly demonstrated that hypoxic preconditioning with H2O2 and CoCl2 can improve the cell proliferation and viability as well as lead to decline in cell apoptosis.48 hours. Then, the MSCs were exposed to lethal dose of H2O2 (300 μM) for 24 hours followed by 24 hours recovery. MTT assay and trypan blue staining were conducted to evaluate the cell proliferation and viability. Also, TUNEL assay was done to study the cell apoptosis. Results: According to our data, cell proliferation in group preconditioned groups increased significantly compared to the other groups after 6, 12, 24 and 48 hours (P< 0.05). Cell survival significantly increased in preconditioned groups with H2O2 and hypoxia after 6, 12, 24 and 48 hours (P< 0.05). Moreover, cell apoptosis was decreased in preconditioned groups significantly than control group after 6, 12, 24 and 48 hours (P< 0.05). Conclusion: This study clearly demonstrated that hypoxic preconditioning with H2O2 and CoCl2 can improve the cell proliferation and viability as well as lead to decline in cell apoptosis.
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نویسندگان مقاله sh خطیبی | sh khatibi


r halabiyan | r halabiyan


a محمدی روشنده | a mohammadi roushandeh


نشانی اینترنتی http://celljournal.org/journal/article/abstract/95
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