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Cell Journal، جلد ۱۷، شماره Suppl ۱، صفحات ۳۸-۳۸
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عنوان انگلیسی Ps-40: Synergistic Effects of Temozolomide and Thymoquinone on U87MG Cell Line
چکیده انگلیسی مقاله Objective: The alkylating agent temozolomide is the major chemotherapeutic drug used clinically in the treatment of glioblastoma multiforme. Glioblastoma is still associated with a poor prognosis, because after first treatment it recurs, and then, displays resistance to further temozolomide treatment. Therefore, resistance is a major obstacle to glioblastoma therapy. Studies show that the combination therapy with natural substances can enhance the anticancer activity of temozolomide. Thymoquinone is a bioactive ingredient from Nigella sativa and has been investigated for its anticancer activities in a variety of cancer cell lines. This study investigated the mechanism behind temozolomide-induced cell death and the possibility that thymoquinone might increase temozolomide efficacy. Materials and Methods: Dose response study was performed to determine the suitable doses of each drug for using in these experiments. U87MG cells were treated with 10, 20, 50 and 100 μM temozolomide and 10, 20, 50, 100, 150 and 200 μM thymoquinone alone, and also in combination of 20 μM temozolomide with 10, 20, 50, 100, 150 and 200 μM thymoquinone for MTT assay. For other tests, concentration of 20 μM temozolomide and/ or 50 μM thymoquinone were chosen. For quantification of apoptosis, necrosis and autophagy, the cells were acridine orange ethidium bromide stained and detection of acidic vesicular organelles was performed. Data were analyzed by one-way ANOVA and P< 0.05 was considered significant. Results: Temozolomide and thymoquinone, alone and in combination, decreased significantly cell viability in U87MG cells, in a concentration and time dependent manner. Temozolomide induced both apoptotic cell death and cytoprotective autophagy which was suppressed by thymoquinone, resulting in a decrease in autophagy and an increase in apoptosis. Conclusion: Autophagic cell death plays a crucial role in the fate of cells after temozolomide treatment, and mechanism of reduction of cell resistance to temozolomide by thymoquinone maybe attribute to blocking of autophagy.
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نویسندگان مقاله m خزایی | m khazaei


r سریری | r sariri


m پژوهی | m pazhouhi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/96
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