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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۱-۱۱
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عنوان انگلیسی Is-11: Large Scale Production of Human Mesenchymal Stromal Cells for Use in The Treatment of Stroke
چکیده انگلیسی مقاله Mesenchymal stromal cells (MSC) are under intensive investigation in regenerative medicine clinical trials and, because of their immunosuppressive properties, in studies to facilitate engraftment of hematopoietic cells and solid organs, and to prevent or treat graft-versushost disease. The cells may be generated from bone marrow, adipose tissue and cord blood, and although their production is relative straightforward, it is labor intensive when using traditional flask-based culture techniques. To address this we have evaluated the Terumo Quantum bioreactor for the preparation of allogeneic MSC to be used for the treatment of stroke. This is a hollow-fiber automated system which is seeded with 25ml whole marrow. The non-adherent cells are removed after ~24 hours and the adherent cells are fed via continuous flow of medium containing human platelet lysate. Growth is monitored by measuring glucose consumption and lactate production. The cells are harvested when the medium flow rate reaches 0.4ml/ min and the lactate level is 4mM. The cells can then be seeded into a second bioreactor for additional expansion. MSC obtained using this method were essentially identical to those grown in tissue culture flasks with respect to immunophenotype, CFU-F production and tri-lineage differentiation. In a rat model of stroke they showed functional activity. The target MSC number of 2x108 cells was obtained in half the number of passages and nine days earlier than when flasks were used for manufacturing. The number of open procedures was reduced from over 50,000 using flasks to 133 using the bioreactor. This manufacturing method was cleared by the US FDA for use in the stroke trial, which is currently accruing patients.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/305
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