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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۶-۱۶
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عنوان انگلیسی Is-16: Collagen Type I Modified Films for Regeneration of Corneal Endothelium
چکیده انگلیسی مقاله Objective: Corneal transplantation, a common surgical protocol for visual acuity improvement, is limited owing to a shortage of high quality donor corneas and/ or its accurate replication of structural and biochemical composition of native cornea in a scaffold. Construction of neo-corneas utilizing novel, biocompatible and biodegradable scaffold source, could address such formidable challenges. Herein, we designed optically transparent, micro-structurally stable poly(lactide-coglycolide) (PLGA) and degummed silk films, surfacemodified with collagen type I as an alternative scaffold source for bioengineering cornea. Materials and Methods: First, silk from Bombyx mori and PLGA (Mw 90,000) were used to fabricate the transparent films, followed by its surface modified using collagen type I. The morphological and structural properties were analyzed by FE-SEM, AFM, FTIR, contact angle, etc. In vitro biological compatibility was studied such as morphology, initial attachment, proliferation, mRNA expression, and proteins related functions using primary corneal endothelial cells. Results: FE-SEM images display the surface roughness, confirming the coating of collagen type I on the respective films. AFM results show collagen type I modified films have a higher Ra (nm) values than unmodified surfaces. Although, collagen type I and cultured cells did not demonstrate any alteration in transparency at visual wavelength, however, material type is able to produce some difference on transparency. Cultured corneal endothelial cells on collagen type Imodified surface showed better initial attachment, proliferation, and expression of mRNAs. Even though differences occur between collagen type I modified films, they do not show any significant differences on morphology, expression of ZO-1 and Na+/K+-ATPase and well-expressed their functional proteins which are regulated functions of corneal endothelium. Conclusion: Overall, the results suggest that collagen type I modified surface of PLGA film and silk fibroin film may be a suitable alternative for high quality corneal tissue expansion and transplantation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/310
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