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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۳۱-۳۱
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عنوان انگلیسی Is-31: High-Performance ZnO Hollow Spheres Based Drug Carriers for Chemo- Photodynamic Cancer Therapy
چکیده انگلیسی مقاله Objective: Development of novel, multimodal therapeutic nanocarriers for ultra-high medicine loading holds paramount promises in cancer diagnosis and therapy paradigm owing to the limitations of conventional chemotherapeutic drugs. Herein, we have developed photodynamic ZnO hollow spheres (ZHS) based therapeutic system for high performance intracellular delivery of anticancer drug (daunorubicin; DNR) in order to enhance drug efficacy per dosage in cancer therapy, followed by evaluation of drug-loading efficiency and synergetic chemo-photodynamic cytotoxic effects. Moreover, zinc oxide nanorods (ZNR) loaded with drug was also studied as a comparative material. Materials and Methods: Low temperature solution grown ZHS was synthesized via two steps process; first, formation of ZnO-Zn(OH)2 shell on the substrate surface; second, another layer of ZnO-Zn(OH)2 is deposited on the step I resulted composite nanospheres surface followed by the formation of ZHS with addition of NH4OH. The ZNR were also synthesized by aqueous solution method at low temperature, followed by their detailed characterizations. Further, daunorubicin drug was loaded into above materials under adequate stirring in dark conditions. The efficiency of ZHS-DNR and ZNR-DNR complex were studied by assessing their in vitro drug release, cellular internalization, DNR localization, cytotoxicity and apoptosis using CLSM microscopy, MTT assay, flow cytometry, etc. Results: The ZHS-DNR complex comprises ultrahigh drug encapsulation efficiency (i.e. 87.7 ± 4.10%) compared to ZNR and previously reported photodynamic materials based drug cargos, which is ascribed to the high-specific surface area, hollow interior, nanostructured wall and good surface permeability. After cellular internalization into human alveolar adenocarcinoma A549 cells, ZnO hollow spheres dissolves in acidic compartments of cancer cells, efficiently releases the loaded drug into lysosomes and successfully destruct cancer cell. Moreover, ZnO hollow spheres behave as a dual-purpose entity that not only serves as a drug carrier but also exhibit a synergetic photodynamic cytotoxic effect. Conclusion: In view of ZnO structures therapeutic potential, the ZHS featured with hollow interior design and inherent photodynamic nature, have proved to be a powerful system as an efficient anticancer and therapeutics delivery agent.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/325
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