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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۳۵-۳۵
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عنوان انگلیسی Ps-3: Comparison of Immunophenotypic Characteristics and Proliferation Potential of Stem Cells Derived from Adult and Deciduous Teeth
چکیده انگلیسی مقاله Objective: To understand whether stem cells derived from adult and deciduous teeth are closely related, we examined immunophenotypic pattern and proliferation potential of dental pulp stem cells (DPSCs) in comparison with cells isolated from human exfoliated deciduous teeth (SCHED). Materials and Methods: Stem cells were isolated from retrieved teeth of donors who signed inform consent by Size-sieved Isolation technique. Expression pattern of hematopoietic (CD34, CD38, CD45 and CD133,), mesenchymal (CD29, CD44, D73, CD146, STRO-1 and CD105) and embryonic (SSEA-4 and OCT-4) stem cells markers was assessed in cultured DPSCs and SCHEDs using flow cytometric analysis. In order to examine cell proliferation, DPSCs and SCHEDs were cultivated for 1, 4, 7, and 10 days and cell proliferation rate was evaluated using the MTT assay. Results: Hematopoietic stem cells markers exhibited a weak expression in both groups (≤2%). More than 98% of the cells in both groups were positive for Mesenchymal surface markers (CD29, CD44, and CD105). Expression of STRO-1, SSEA4, and OCT-4 were significantly higher in SCHED. Proliferation rate of DPSCs and SCHED showed no significant difference up to day 4, however significantly higher expansion rate of SCHEDs was shown compared to DPSCs at days 7th and 10th. Conclusion: Both stem cells expressed common MSC markers. Permanent and deciduoustooth germs are derived from the ectoderm of the first branchial arch and the ectomesenchyme of the neural crest, however, different levels of expression of STRO-1, SSEA4, and OCT-4 suggests there might be more than one stem cell population existing within these tissues which differ in their embryonic status, and SCHEDs are a more primitive stem cell population in comparison to DPSCs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/329
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