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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۳۶-۳۶
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عنوان انگلیسی Ps-4: Potential of Chicken Feet Collagen as Scaffold for Tissue Engineering Applications
چکیده انگلیسی مقاله Objective: Evaluation and characterization of chicken feet collagen and it’s gel for in vitro proliferation of stem cells. Materials and Methods: RPMI 1640 was purchased from Invitrogen included 10% (v/v) fetal bovine serum (FBS, Gibco) and 1,00 U penicillin streptomycin/ ml were used as a medium. Hydrochloric acid (HCl 0.055 M) and pepsin (Sigma) 5% was used to extract the pepsin-solubilized collagen (PSC) from chicken feet for 40 hours. Derived collagen was characterized by using electrophoresis and the fourier transform infrared (FTIR) as well as X- ray diffraction (XRD) assay. Collagen gel was then prepared with 30 mg/ml concentration and tested for mechanical strength by Zwick/roell Z010. Fibroblast cells (Pasteur, Iran) were seeded at 1×106 cells/ml in collagen gel for study of cell behaviors. Results: In electrophoresis pattern, three subunits (β, α1, α2) were observed, that was the same as bovine skin collagen. Indeed, band intensity of α1 and α2 chains were the same, suggesting collagen obtained from chicken feet has more than two types of collagens. Also, FTIR spectra demonstrated a similar pattern for collagens of two sources. XRD assay confirmed that there is hydroxyapatite component in the chicken feet collagen. Mechanical strengths of 31.2 and 3.3 KPa were observed for the bovine skin and chicken feet collagen gels, respectively. However it can be used for cell seeding and tissue engineering. Fibroblast cells could also proliferate well inside the chicken feet collagen gel with 10 times increase after 12 days. Conclusion: Collagen extracted from chicken feet has potential to be used for tissue engineering applications as scaffold particularly for bone tissues due to the existence of hydroxyapatite in the extracted collagen.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/330
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