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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۰-۴۰
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عنوان انگلیسی Ps-8: Isolation, Culture and Characterization of Canine Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Isolation and biological characterization of bone marrow derived mesenchymal stem cells (BMMSCs) is the first step for cell therapy of different human and animal diseases. The objectives of the present study were isolation, culture and characterization of canine BM-MSCs. Materials and Methods: Two clinically healthy large mixed breed dogs of 1 and 3-year-old were used. Bone marrow (20 ml) was collected from the proximal humerus under general anesthesia and cultured and passaged in αMEM and FBS. Cells of passages 4, 5, and 8 from older dog and passage 8 from younger one were seeded in 24-well plates at a density of approximately 5×104 cells per well, for 8 days and three wells per day were used to determine cell number. The population doubling time (PDT) at each passage was calculated. Karyotyping of cells of passages 1 and 8 were performed. The chromosome numbers were counted and evaluated to obtain the percentage of diploid cells. The mean and SE of counted cells in growth curve analysis were compared using independent sample t test and one way ANOVA (SPSS for Windows, version 11.5, p≤0.01). Results: BM-MSCs displayed fibroblastic-like morphology in all passages. The PDT of the passage 8 of younger dog was 41.1 hours and of the passages 4, 5, and 8 of older dog were 65.8, 55.3 and 90 hours, respectively. Proliferation of canine MSCs in younger donor was higher than adult donor. The chromosome number of the canine BM-MSCs was 2n=78 and the results showed that the BM-MSCs were normal. Conclusion: The increase in the PDT of BM-MSCs occurs with the increase in age. Karyotyping of these cells was normal. These cells can be used for tissue repair and regeneration in most organs in medicine and veterinary medicine.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/334
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