این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۱-۴۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-9: Hypoxia Preconditioning Improved Survival of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: In the recent decade, human umbilical cord Blood derived mesenchymal stem cells (hUCB-MSCs) provide enormous potential for appropriate therapy in different fields. Researches have shown that hypoxia preconditioning (HP) of bone marrow derived Mesenchymal stem cells ( BM -MSCS) enhance their survival and extend their life span and inhibit pro inflammatory chemokines in MSCS. In this study, we examined the proliferation capacity of hUCB-MSCs under hypoxic condition in comparison with normoxic status. Materials and Methods: HUCB-MSCs were isolated. The Ethical approval was obtained from the institutional ethical review board at Blood transfusion Research Center. The isolated population have been characterized as mesenchymal stem cells by surface markers analysis and functional properties. UCBMSCs were exposed to HP protocol consisted on 2 sets of hypoxia (15 minutes, 2.5% O2) and re-oxygenation (30 minutes, 21% O2), prior to hypoxic challenge (72 hours). Cell survival was measured by the trypan blue staining. Apoptosis was measured with MTT assay. Doubling time and doubling rate were calculated in hypoxic and normoxic groups. Results: The results showed that HP status increases cell proliferation of UCB-MSCS (1.2 fold) in comparison with control group. The MTT results showed that cell viability of hUCB-MSCs after 72 hours of hypoxia preconditioning significantly increased (0.36 ± 0.07) in comparison with normoxic group (0.26 ± 0.07) (p< 0.05). Conclusion: Our results suggested that hypoxia preconditioning - UCB-MSCS could be a potential therapeutic avenue for increasing the efficacy of stem cell therapy and support future application of UCB-MSCS for regenerative medicine.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/335
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات