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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۲-۴۲
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عنوان انگلیسی Ps-10: Characteristics of CD146 Positive Portion of Pulp Polyp Derived Stem Cells
چکیده انگلیسی مقاله Objective: Pulp polyps (chronic hyperplastic pulpitis) are pathological tissues produced in an inflammatory proliferative response within a tooth. We have previously isolated stem cells from them, the “Pulp Polyp Stem Cells (PPSCs)”. PPSCs’ cultures showed in heterogeneous phenotype with a low stem cell yielding rate. In the present study we evaluated the effect of CD146 isolation on the isolation of PPSCs. Materials and Methods: Single cells from enzymatically digested pulp polyp tissues underwent CD146 isolation using magnetic cell sorting technique. The positive and negative portions were cultured and their morphological features, flowcytometric antigen panel and differentiation capacity was compared. Results: Pulp polyp CD146 positive cells displayed spindle shape morphology with successful differentiation into adipocytes and osteoblasts. They also expressed key antigen markers such as STRO-1, CD73, CD90, and CD105. CD146 positive portion of the polyp derived cells formed an average of 90 ± 30.6 which was significantly higher than the negative counterpart with no colonies (p = 0.014) Conclusion: The CD146 positive cells isolated from pulp polyps fulfill the criteria for Mesenchymal stem cell definition, while the negative portion could not. The results of this study showed that CD146 enrichment can more homogenized the cultures of PPSCs and the stem cell portion is restricted to positive portion of cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/336
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