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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۳-۴۳
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عنوان انگلیسی Ps-11: Myeloid Leukemia Cells Exhibit Higher Amounts of Telomerase Alternative Splicing Variants Compared to Hematopoietic Stem Cells
چکیده انگلیسی مقاله Objective: Telomerase activity control has great importance in cancer research. There is a controversy about the role of alternative splicing of human telomerase (hTERT), the catalytic subunit of telomerase enzyme, in regulation of telomerase activity. We aimed to find any difference between hTERT Alternative Splicing Variants (ASV) as specific targets which may differ between normal hematopoietic cells and leukemia cells. Materials and Methods: Hematopoietic Stem Cells (HSCs) were isolated using a Fluorescent activating cell sorting (FACS) technique. CD34+CD38- cells were considered as long term HSCs. Acute myeloid leukemia cell line KG-1 were cultured and detached enzymatically. Quantitative amounts of four different ASV mRNAs of hTERT including full length, alpha deletion, beta deletion and alpha beta deletion was measured using Real Time PCR. Results: Flowcytometric analysis showed successful isolation of HSCs. These cells expressed low amounts of hTERT mRNAs with equal frequency in all variants. KG-1 cancer cells showed large amounts of hTERT mRNAs with higher percentage of alpha variants. Conclusion: In this study we have clearly showed that myeloid leukemia cells express higher amounts of telomerase ASV mRNAs compared to normal HSCs but there was no trend toward a specific variant. This can be one of the reasons of unlimited replicative potentials for these cells. However, alternative variants of this gene are not useful targets for differential therapy of cancerous cells without affecting normal HSCs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/337
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