این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۴-۴۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-12: Stem Cell Properties are Restricted to CD146 Positive Portion of Dental Pulp Derived Cultured Cells
چکیده انگلیسی مقاله Objective: Routinely, cultures of dental pulp stem cells (DPSCs) are achieved by direct culturing of tissue derived single cell suspensions which are very heterogeneous and contaminated by fibroblasts. The purpose of this study is to examine that CD146 expression can discriminate between dental pulp fibroblasts (DPFs) and DPSCs. Materials and Methods: Based on the CD146 expression, magnetic cell sorting was done to purify DPFs and DPSCs from cultures of pulp derived cells. Colony forming assay, differentiation, and flowcytometry was performed to characterize the cells. Results: While every 10,000 of CD146 positive cells formed 96 ± 18.7 colonies, CD146 negative portion could not form any colonies (p< 0.001). In addition, only the CD146 positive cells could differentiate to adipocytes or osteoblasts. Staining and RT-PCR confirmed these results. Conclusion: Our results reveal that only the CD146 positive portion of the dental pulp derived cells were able to form colonies and differentiate into osteoblasts or adipocytes and are thus true stem cells, while the CD146 negative cells failed to do so. CD146 expression can be considered as a discriminative factor between DPSCs and DPFs.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/338
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات