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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۵-۴۵
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عنوان انگلیسی Ps-13: Expression of Telomerase Alternative Splicing Variants Doesn’t Change during Early Stages of Hematopoiesis
چکیده انگلیسی مقاله Objective: Telomerase activity has great importance in maintenance of self renewality. There is a controversy about the role of alternative splicing of hTERT, the catalytic subunit of telomerase enzyme, in regulation of telomerase activity. We aimed to find any changes in hTERT alternative splicing variants during early stages of normal hematopoiesis as a regulator in diminishing the self renewality within more descendant progenies. Materials and Methods: Hematopoietic stem cells (HSCs) were isolated using a fluorescent activating cell sorting (FACS) technique from bone marrow. CD133+CD34- cells were considered as pluripotent stem cells, CD34+CD38- cells as long term (LT)-HSCs and CD34+CD38- cells as short term (ST)-HSCs. Quantitative amounts of four different Alternative splicing variant mRNAs of human telomerase (hTERT) including full length, alpha deletion, beta deletion and alpha beta deletion was measured using real time PCR. Results: Flowcytometric analysis showed successful isolation of HSCs subsets. These cells expressed low amounts of hTERT mRNAs with equal frequency in all variants. There was only a slight elevation in the frequency of hTERT mRNA variants in the SH-HSCs. Conclusion: In this study we recapitulated previous finding that HSCs show only slight amounts of telomerase mRNA with a step up from LT-HSCs to ST-HSCs. However, we could not find any specific alternative splicing variant mRNAs as a dominant variant and the pattern of expression of these variants didn’t change during early stages of hematopoiesis. It can be conclude that alternative splicing does not have a significant role in regulation of telomerase activity in normal hematopoietic system.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/339
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