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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۶-۴۶
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عنوان انگلیسی Ps-14: Osteogenesis Induced by Nanofiber Scaffold in Bone Marrow Mesenchymal Stem Cells via Inhibition of Transforming Growth Factor-Beta Signaling Pathway
چکیده انگلیسی مقاله Objective: One of the most promising ways to repair critical-size bone defects, is the fabrication of nanofiber scaffolds which are designed to mimic the structure of the natural bone tissue. Materials and Methods: In this study, electrospinning technique was performed and poly L lactide nanofiber (PLLA) and nano hydroxyapatite coated- poly L lactide nanofiber (PLLA/nHA) were constructed. Then the effect of these scaffolds on the osteogenic differentiation of human bone marrow mesenchymal stem cells (BM-MSCs) was investigated in vitro under osteogenic induction in comparison with tissue culture plates (TCPs). Furthermore, Transforming growth factor beta1 (TGF- beta) signaling pathway activity has been analyzed as an important regulatory pathway of osteogenesis. For this purpose, TGF beta1 recombinant protein and SB431542 small molecule were used as inductive and inhibitory factors of this pathway respectively. After 7, 14 and 21 days, Alkaline phosphatase enzyme (ALP) activity and extracellular calcium deposition were measured. Results: The results showed that cultured BM-MSCs on scaffolds, had higher ALP activity and calcium deposition in compare with TCPs substrates. Meanwhile PLLA/nHA scaffold was the most influential among the all groups. Furthermore, the inhibition of TGF beta signaling pathway by SB431542, led to the increase of ALP activity and calcium deposition and the induction of this pathway by TGF beta1 caused the decrease of ALP activity and calcium deposition. Conclusion: These results suggest that scaffolds could influence intracellular signaling pathways by providing 3D substrate for cells, thus direct cell fate.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/340
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