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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۴۷-۴۷
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عنوان انگلیسی Ps-15: The Role of Embryonic SertoliCells in Maintenance of Spermatogonial Stem Cells
چکیده انگلیسی مقاله Objective: Sertoli cells are unique somatic cells in testis and have close contact with spermatogonial stem cells (SSCs). Adult and embryonic Sertoli cells differ from significant ways. Structural and growth factors secretion changes occur in Sertoli cells as they mature. Our objective was to use embryonic Sertoli cells as a feeder layer for Spermatogonial stem cell maintenance. Materials and Methods: Embryonic Sertoli cells were obtained from E14.5 testis. The isolated cells expressed vimentin in their cytoplasm. SSCs were isolated from adult male mice by enzymatic digestion and cultured on embryonic Sertoli cells for 1 and 2 weeks. After culturing, the expression level of MVH, ZBTB and C-kit were studied by real time-PCR. Then, SSCs were transplanted to azoospermic mice. After 2 months, the recipient testes were examined for spermatogenesis restoration. Results: Our results showed that this co-culture system could increase the expression level of MVH and ZBTB, but decreases C-kit expression. After transplantation, the co-cultured SSCs could restore spermatogenesis to azoospermic mice model. Conclusion: Our findings revealed that embryonic Sertoli cells could maintain SSCs in undifferentiated state in vitro. This co-culture model could improve the SSCs quality in long term culture and restoration of spermatogenesis in vivo.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/341
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