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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۵۰-۵۰
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عنوان انگلیسی Ps-18: Investigation of Endothelial Cells Detachment under The Fluid Flow Shear Stress in A Parallel Plate Hollow Fiber Bioreactor
چکیده انگلیسی مقاله Objective: One of the problems of the oxygenator device which is used during surgical procedures is thrombus formation on polymeric fibers after few hours of usage. Endothelialization of blood-contacting surfaces is one prominent solution for avoiding thrombus formation. However, developing a stable layer of endothelial cells under flow shear stress is an obstacle. In this research different ranges of shear stresses were computed using the computational fluid dynamics (CFD) procedure and then percentage of cell detachment under obtained fluid flow shears have been examined by using a simple parallel plate bioreactor. Materials and Methods: COMSOL software was used for computing the shear stress applied on ECs by fluid flow. Human Umbilical Vein Endothelial Cells (HUVEC, NCBI Code: 554) were obtained from National Cell Bank of Iran. The surfaces of Silicon fiber with 0.6 mm ID (kindly donated by Raumedic, Germany) were modified with collagen type І solution. Different magnitudes of shear stresses were applied on cultured ECs on the outer surfaces of the hollow fiber in a bioreactor. The percent of dislodged cells from the fiber were measured by MTT assay. Results: The experimental results demonstrated that by applying the shear stress of 0.03 and 0.09 dyn/cm2 on cells none of them, shear stress of 0.17 dyn/cm2, 6.6%, shear stress of 0.76 dyn/cm2, 18% and shear stress of 2 dyn/cm2, 33% of the cells were dislodged from the hollow fiber. Conclusion: The results showed that this parallel plate bioreactor can help us to have a uniform shear stress on cells throughout the length of the fiber. For strengthening the cells attachment to the fiber outer surfaces, some improved surface modification methods have to be performed on the fiber. In this way, this approach can be used for oxygenators in the near future.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/344
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