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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۵۱-۵۱
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عنوان انگلیسی Ps-19: The Effect of Avocado/Soybean Unsaponifiables (Piascledine) on Viability of Human Articular Chondrocytes and Adipose Derived Stem Cells
چکیده انگلیسی مقاله Objective: Avocado/soybean unsaponifiables (piascledine) are plant extracts that have been reported to stimulate secretion of extracellular matrix in osteoarticular diseases. So far, the effects of this composition on stem cells and differentiation have not been investigated. In this study the effect of piascledine on the viability of articular chondrocytes and adipose derived stem cells (ADSCs) cultured by micromass method were investigated to determine suitable dose for chondrogenesis of ADSCs and chondrocytes proliferation. Materials and Methods: Articular chondrocytes and ADSCs were harvested in second passage (P2) and third passage (P3), respectively. In micromass culture,aliquots of 2×105 cells in droplets (12.5 μl) were placed on each well in 24-well tissue culture plates. Different doses of piascledine include 1, 5 and 10 μg/ml prepared with both differentiation and nondifferentiation media. MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay was performed on days 4 and 7. Results: The data indicated that concentrations of 1 μg/ml in both differentiation and nondifferentiation media on day 4, and 5 μg/ml in differentiation medium on day 7 are the suitable doses of piascledine for articular chondrocytes viability and proliferation. The best doses of this drug for viability and proliferation of ADSCs were obtained as 10 μg/ml in nondifferentiation medium on day 4, and 5 μg/ml in differentiation medium on days 4 and 7. Conclusion: Piascledine treatment caused to promote viability and proliferation of both articular chondrocytes and ADSCs compared to controls. Also the 5 μg/ml concentration of this drug was suggested for future studies on cartilage extracellular matrix production by ADSCs.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/345
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