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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۵۲-۵۲
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عنوان انگلیسی Ps-20: Evaluation of Nanofiber Scaffold Poly (Є-caprolactone)/Gelatin Biocompatibility in Proliferation of Bone Marrow Mesenchymal Cells
چکیده انگلیسی مقاله Objective: Tissue engineering is an interdisciplinary science that is based on the use of polymer scaffolds along with cells for the regeneration of defective tissues. Considering nano-meter size of the natural extracellular matrix components such as cavities and the diameter of their constitutive fibers, today's interest has been raised to utilizing nanofibrous scaffolds for tissue engineering. As well as the use of stem cells for tissue engineering has great importance due to the ability of these cells to differentiate into different cell types. Materials and Methods: In this study, poly (Є-caprolactone)/gelatin nanofiber scaffolds were prepared and examined for growth and morphology of bone marrow derived mesenchymal stem cells on the scaffolds. In order to prepare nanofiber scaffold, poly (Є-caprolactone) and gelatin were mixed at a weight ratio of 70/30,dissolved in hexa flouro isopropanol (HFP) and electrospun.The bone marrow stem cells were seeded on nanofiber scaffolds and tissue culture plates (TCP) as control. MTT test was used to compare the growth and proliferation of cells on nanofiber scaffolds and TCP. The morphology of cells on nanofibers were also evaluated by SEM. Results: Our results showed an average fiber diameter of 189 ± 56 nm for the nanofibrous scaffolds. The proliferation of cells on PCL/gel nanofibrous scaffolds was found to be higher than that on TCP, indicating that the PCL/gel scaffolds serve as a suitable substrate for cell proliferation. SEM results also showed cell spreading on the nanofibrous scaffolds which is consistent with MTT results. Conclusion: The overall results of this study suggest suitability of PCL/gel nanofiber scaffold for growth and expansion of mesenchymal stem cells from bone marrow. Regarding to the expression of neurotrophic factors by these cells, nanofibrous scaffolds seeded with these cells can have a significant potential in the regeneration of damaged nerve tissue.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/346
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