این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۵۵-۵۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-23: A Modified Stirred Tank Bioreactor for Expansion of Mock Hematopoietic Stem Cells
چکیده انگلیسی مقاله Objective: Evaluate a performance of Modified Stirred Tank Bioreactor (MSTB) with low shear stress and simple appliance for ex vivo Expansion of Hematopoietic Stem Cells using hematopoietic cell line (U937). Materials and Methods: RPMI 1640 was purchased from Invitrogen included 10% (v/v) fetal bovine serum (FBS, Gibco), 1,00 U penicillin streptomycin/ml were used as a medium. U937 cell line (Invitrogen) as mock HSCs were used to inoculate static and dynamic culture systems. Modified stirred tank bioreactor (MSTB) System fabricated in Sahand University of Technology was used as dynamic system. Cell surface marker CD14 antigen was detected using reagents. The treated and/ or untreated cells were incubated simultaneously with fluorescein isothiocyanate (FITC)-conjugated CD14 monoclonal antibody (Invitrogen). After washing, at least 104 cells were analyzed by flow cytometry (Becton- Dickinson, NJ, USA).The morphology of cells was also evaluated quantitatively by a parameter named cell roundness, Rc as described elsewhere. The cell roundness, Rc=2(Ac) 0.5/lc, was determined with area, Ac, and peripheral length, lc, of each cell by image processing software (Belview). Results: The U937 cell as mock HSCs showed about 97.5 ± 1.7 and 80 ± 2.8-fold for MSTB and T-flasks respectively. The almost all cells proliferated in the Tflasks during the 8-day culture period were CD14- cells 93.7%. The percentage of CD14- cells, however, decreased to 73% in the cultures with MSTB system at the same culture period. The frequencies of round-shaped cells (Rc > 0.9) for the T-flask and MSTB cultures were 92.17% ± 2.1 and 68% ± 4.9 respectively. The MSTB system shows an acceptable performance in this regard due to providing lower shear stress on the cells. Conclusion: In this work, a modified stirred tank bioreactor system was introduced for possible use in HSCs expansion in vitro with reducing shear stress exerted on the cells. U937 cells showed the considerable increase in the cell proliferation when they were cultured in the modified bioreactor, as compared to the cultures in Tflask. Majority of the cells cultured in the T-flask and MSTB were the round-shaped and CD14- cells after 8 days. The present study showed the potential of the MSTB to use in the expansion of suspension culture of stem cells in vitro such as HSCs.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/349
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات