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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۶۳-۶۳
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عنوان انگلیسی Ps-31: Presence of Dental Mesenchymal Stem Cells in Oral Reactive Lesions by Immunofluorecence Method
چکیده انگلیسی مقاله Objective: Stem cells are innovative tools for tissue regeneration. Several niches in adult human body are colonized by multipotent stem cells but access to these potential reservoirs is often a limiting point. Although human dental pulp stem cells isolated from healthy teeth have been extensively characterized, it is unknown whether stem cells also exist in the reactive lesions of oral cavity such as pyogenic granuloma and peripheral ossifying fibroma which are kind of inflammatory proliferation of different cell families. Here we explored whether cells retrieved from such lesions have dental mesenchymal stem cell (DMSCs) or not. Materials and Methods: Two pyogenic granulomas and two peripheral ossifying fibromas were achieved through excisional biopsy and preserved in PBS-EDTA in -86°C. Then we cut them in 5μm diameter with Cryostat. After washing these samples with PBS we used anti-STRO-1 primary antibody that is a monoclonal IgM derived from mice; after that we applied secondary antibody that was goat anti-mouse IgM-FITC to detect STRO-1+ cells by immunoflourescence technique. Results: Immunofluorscence microscopy revealed presence of STRO-1+ cells in these lesions, particularly localized at perivascular zone. The negative control group wasn't glowing. Conclusion: Based on these results, it was found that reactive lesions of pyogenic granuloma and peripheral ossifying fibroma have STRO-1 positive mesenchymal cells, which raises the possibility that these cells can be used as a new, accessible and non-invasive source of stem cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/357
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