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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۶۶-۶۶
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عنوان انگلیسی Ps-34: Effect of Crocin on Differentiation of Neural Stem Cells to Oligodendrocytes
چکیده انگلیسی مقاله Objective: Oligodendrocytes are myelin forming cells that develop from proliferating Oligodendrocytes precursor cells (OPCs), and damaged in multiple sclerosis (MS) disease. Recent studies focus on neuroprotective factors that promote neural stem cells differentiation to Oligodendrocytes for the aim of cell therapy in MS patients. Crocin is the main carotenoid of saffron that has neuroprotective effects. In current study, we evaluated how it might differentiate neural stem cells to oligodendrocytes. Materials and Methods: In this study, neural stem cells were isolated from embryos cortex of E14 pregnant rat. Optimum concentration of crocin extract was determined using alamar blue. Neural stem Cells (NSCs) were divided into 3 groups and treated with Media (DMEM- F12): The first group treated with bFGF (10ng/ml), the second group : bFGF(10ng/ml) and PDGF-AA(30 ng/ml) and the third group: 100 mM of crocin solution for 24 hours, 4 and 6 days. The expression of OLIG2 marker as early OPCs marker was assayed by flowcytometry technique. The level of SOX10 (a transcription factor in differentiation of neural cells) mRNA was evaluated by RT-PCR. MBP (myelin basic protein) expression was compared between different groups in the late stages of differentiation. The positive control for MBP was PDGF-AA (30 ng/ml) + laminin (1 ng/ml). Results: Flowcytometrys data showed the expression of olig2 in crocin group (96%) is similar to positive control group (98%). According to RT-PCR results the expression of sox10 in two above groups was the same. Conclusion: Our data demonstrates that crocin has potent role on olig2 and sox10 expression, but not effective improvement on MBP marker. Therefore, crocin could stimulate early neural stem cells differentiation to OPCS (oligodendrocyte precursor cells).
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/360
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