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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۶۸-۶۸
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عنوان انگلیسی Ps-36: Expression of Drug Metabolism Enzymes in Hepatocyte-Like Cells Derived from Menstrual Blood Stem Cells
چکیده انگلیسی مقاله Objective: Recently, valuable characteristics of menstrual blood stem cells (MenSCs) have impelled scientists to take its advantages for cell therapy of different diseases including liver disorders. In this study, we examined mRNA expression levels of drug metabolizing factors including glutathione S-transferase (GST) and cytochrome P- 450 (CYP) enzymes in differentiated hepatocyte-like cells from MenSCs. Materials and Methods: The isolated MenSCs were characterized and differentiated into hepatocyte-like cells using hepatocyte growth factor (HGF) and oncostatin M (OSM) in combination with other components in serum-free culture media. After primary characterization of hepatocyte markers such as albumin (ALB) and tyrosine aminotransferase (TAT), mRNA expression of GSTA1, GSTA2, GSTP1, CYP3A4 and CYP7A1 were assessed in differentiated cells in reference to undifferentiated cells using real-time PCR. Results: Based on immunofluorescent staining and Real-Time PCR data, the differentiated MenSCs could express hepatocyte markers like ALB and TAT at mRNA and protein levels suggesting development of hepatocyte-like cells from MenSCs. Moreover, the expression levels of GSTA1, GSTA2 and CYP3A4 mRNA were up-regulated in differentiated cells compared to undifferentiated cells. The expression of CYP7A1 gene was also remarkable on the last day of differentiation process. Although the expression level of GSTP1 also elevated during differentiation, it was not statistically significant (p=0.6). Conclusion: Based on accumulative data, MenSCs could be viewed as an accessible population of stem cells with differentiation ability into drug-metabolizing hepatocyte-like cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/362
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