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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۶۹-۶۹
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عنوان انگلیسی Ps-37: The Expression of NPPA Splice Variants during Cardiac Differentiation of Mouse Mesenchymal and Embryonic Stem Cells
چکیده انگلیسی مقاله Objective: NPPA is an early and specific marker for functional myocardium of the embryonic heart. NPPA gene encodes for a precursor of atrial natriuretic peptide (ANP). The expression pattern of natriuretic peptide precursor A (NPPA) gene during development of cardiac chamber is highly dynamic. Moreover, the expression profile of NPPA gene is different between human and mouse. So far, three alternatively spliced variants have been reported for NPPA gene in human. In mouse, no alternatively spliced isoform of NPPA gene has been reported. In the current study, we studied the expression of NPPA gene during cardiac differentiation in vitro and in vivo. Materials and Methods: Cardiac differentiation of the ADSCs was induced by 5-azacytidine, bone morphogenetic protein-4 (BMP4) or coculture with the mouse cardiomyocytes, and ES cells were differentiated at the presence or absence of bone morphogenetic protein-4 (BMP4). To assess the expression of NPPA splice variants during heart development, the cardiac area of 8-day mouse embryos and one-week old mice were isolated and used for evaluation. Results: Two-week differentiated ADSCs and ES cells expressed some cardiac-specific makers, including atrial natriuretic peptide (ANP). Three additional intron-retained splice variants of NPPA were also detected during cardiac differentiation of the ADSCs and ES cells. In addition, we detected three intronretained splice variants of NPPA in 8-day mouse embryonic heart. In the mature cardiomyocytes of 1-week old mice, only the correctly spliced isoform of NPPA gene was expressed. Freshly isolated stromal vascular fraction also expressed one intron-retained isoform of NPPA gene. Conclusion: Our findings have provided evidence for the expression of intron-retained splices of NPPA mRNA during the early stages of mouse cardiogenesis as well as in the mouse adipose tissue. However, the functional significance of these variants remains to be investigated.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/363
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