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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۷۰-۷۰
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عنوان انگلیسی Ps-38: The Effects of Pre- and Post- Ischemic Neural Stem Cell Intra- Ventricular Injection on Brain Stroke in Rats
چکیده انگلیسی مقاله Objective: Brain stroke, the second most important cause of mortality in developed countries, does not have an effective treatment yet. Moreover, clinical outcome after stroke is not satisfactory and some patients remain disabled. In some cases such as surgeries, the patients face the risk of brain stroke. Nowadays, cell therapy is a new approach to establish a treatment for brain stroke. Neural stem cells are one of the good resources that can regenerate the central nervous system regarding their ability to differentiate into 3 neural lineages, including neurons, oligodendrocytes and astrocytes. Materials and Methods: Neural stem cells were isolated from rat embryo, and then expanded until passage 4. The neural stem cells expressed Nestin and were differentiated to neurons, oligodendrocytes and astrocytes by adding 5% fetal bovine serum to their culture media. The isolated neural stem cells were labeled with PKH26 and injected into lateral ventricle of rat brains by stereotactic method in 2 groups: first group received the cells one day prior to brain stroke; the second one received the cells one day after the brain stroke. In control group, PBS was injected. HandE staining was performed to determine the necrosis and ischemic area. Results: Neurological assessment shows that clinical improvement in second group is better than the first one after 28 days. Also, tracking the neural stem cells by PKH26 indicates that the cells show a preference to migrate more to the cortex and the striatum in comparison with the other areas of the brain in both groups. HandE staining expresses post-ischemic injection (2nd group) has the least necrosis and ischemic area and the control group has more ischemic and necrosis areas than the other groups. Conclusion: Post-ischemic neural stem cell injection has better effects than pre-ischemic injection. Also, the injection of neural stem cells reduces the necrosis and the ischemic area according to the ability of these cells to regenerate the central nervous system.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/364
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