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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۷۱-۷۱
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عنوان انگلیسی Ps-39: Quantitative Proteomics Analysis Highlights The Role of Redox Hemostasis and Energy Metabolism in Human Embryonic Stem Cell Differentiation to Neural Cells
چکیده انگلیسی مقاله Objective: Neural differentiation of human embryonic stem cells (hESCs) is a unique opportunity for in vitro analyses of neurogenesis in humans. Extrinsic cues through neural plate formation are well described in the hESCs although intracellular mechanisms underlying neural development are largely unknown. Proteome analysis of hESCs differentiation to neural cells will help to further define molecular mechanisms involved in neurogenesis in humans. Materials and Methods: Using a two-dimensional differential gel electrophoresis (2D-DIGE) coupled mass-spectrometry system, we analyzed the proteome of hESCs differentiation to neurons at three stages, early neural differentiation, neural ectoderm and mature neurons. Results: Out of 137 differentially accumulated protein spots, 118 spots were identified using MALDITOF/ TOF and LC MS/MS. We observed that proteins involved in redox hemostasis, vitamin and energy metabolism and ubiquitin dependent proteolysis were more abundant in differentiated cells, whereas the abundance of proteins associated with RNA processing and protein folding was higher in hESCs. Higher abundance of proteins involved in maintaining cellular redox state suggests the importance of redox hemostasis in neural differentiation. Furthermore, our results support the concept of a coupling mechanism between neuronal activity and glucose utilization. The protein network analysis showed that the majority of the interacting proteins were associated with the cell cycle and cellular proliferation. Conclusion: These results enhanced our understanding of the molecular dynamics that underlie neural commitment and differentiation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/365
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