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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۷۷-۷۷
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عنوان انگلیسی Ps-45: Evaluation of The VE-cadherin Gene Expression in Adipose Derived Stem Cells and Purified Mesenchymal Stem Cells under Chemical and Mechanical Stimuli
چکیده انگلیسی مقاله Objective: Adipose-derived stem cells (ADSCs) are a heterogeneous cell population that may be enriched by positive selection with antibodies against CD271, yielding a selective cell universe with higher proliferation and differentiation potential. ADSC cells can sense their surrounding biochemical and biophysical factors, which play major roles in their differentiation toward different phenotypes. The scope of the current study is to evaluate and acquire the best endothelial differentiation yielding through purification of ADSCs using specific CD271 cell marker and compare their results with that of unpurified ADSCs both exposed under chemical and mechanical conditions in vitro.Thus, we examined endothelial differentiation of neat ADSC and ADSCCD271+ cells in response to specific growth factors, shear force and combination of these two factors. Materials and Methods: MSCs were isolated from fat tissue and cultured in DMEM containing 10% FBS. Then they were characterized in passage 2, using flow cytometery. CD271 expression level was examined and purified using flow cytometery sorting. ADSCs-CD271+ were cultured and characterized and their expression level was examined. ADSCs and ADSCs-CD271+ were cultured in collagen coated-tubular silicone scaffolds and then exposed to VEGE (for 7 days), shear stress (in a designed perfusion bioreactor, under application of shear stress for 24 hours) and combination of these two signals. Real-Time PCR was used to quantify the expression level of the VE-cadherin gene (the factor specifying endothelial cells) in the experimental groups. Results: 1% of ADSCs showed CD271 marker, which was increased to 74% after purification. Evaluation of surface markers using flow cytometry confirmed the mesenchymal characteristics of both cell groups. According to the results of Real-time PCR, the chemicalmechanical group, the mechanical group and then the chemical group (in both purified and unpurified cells) compared to their corresponding control group, showed the highest expression of VE-cadherin, respectively. Moreover, the expression of VE-cadherin was generally higher in ADSCs-CD271+ groups compared to results in ADSCs ones. Conclusion: The findings of this study demonstrate that MSCs purification as well as combining the chemical and mechanical factors can improve VE-cadherin expression.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/371
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