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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۷۹-۷۹
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عنوان انگلیسی Ps-47: Platelet Lysate as An Alternative Supplement for Growth and Expansion of Human Bone Marrow Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Despite the fact that there are huge capabilities for human mesenchymal stem cells (MSCs) to differentiate into various tissues, the major obstacle for using these cells in clinic is the presence of fetal bovine serum (FBS) that prohibits basically in human studies due to ethical issue and transferring animal diseases. For overcoming this drawback, many alternatives have been tested such as platelet lysate (PL). Materials and Methods: Our main strategy was substation of animal sera using typically for cultivation of MSCs in vitro by umbilical cord blood-derived PL. In brief, two separate category of mesenchymal stem cells was grown in MEM-alpha medium supplemented by either10% fetal calf serum or PL in ascending concentrations: 5, 10, and 20%. After >90% of confluence, mesenchymal stem cells were evaluated regarding surface markers, Adipo- and osteogenic differentiation, and cytotoxicity of PL. Also, doubling time and growth kinetics of two cell categories was calculated by a mathematic formula. Results: MSCs in PL-supplemented medium have a significant expression of CD105, CD73, and CD29, but not in case of CD90, CD45/CD34, and CD31. PL groups have similar behavior compared with FBS group for differentiating toward adipocyte or osteocyte. Only 5% PL group were proliferated in parallel of FBS group with a bit longer doubling time while 10%, and 20% PL groups did not have any markedly expansion. In exception of 20% PL, we observed no cytotoxic effect for MSCs. Conclusion: Platelet-derived products like as PL have been used widely for the expansion and differentiation of MSCs in clinical trials. However, it requires to test some different concentrations of PL for achieving the best compared with FBS. In the overall analysis, we found 5% PL obtained from umbilical cord blood can make a suitable serum-free media for MSCs will be used in the cell therapy centers.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/373
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