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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۸۱-۸۱
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عنوان انگلیسی Ps-49: Dexamethasone, Low Intensity Ultrasound and Osteogenic Differentiation of Adult Stem Cells
چکیده انگلیسی مقاله Objective: In this study, we investigated the combination effect of Dexamethasone (Dexa) and Low- Intensity Ultrasound (LIUS) on osteogenic differentiation of rat bone-marrow derived mesenchymal stem cell (rMSCs). Our hypothesis was synergistic effect of tow induction materials in osteogenic differentiation on rMSCs. Materials and Methods: Ultrasound instrument with 3MHz frequency was calibrated by radiation force method. rMSCs isolated from rat’s tibia and femur were cultivated. Cells promoted for three successive passages in order to purify them. Passage three rMSCs were grouped in 10-8mMol Dexa and LIUS and just Dexa. The LIUS and Dexa group recived stimulation at 3MHa frequency and 355mW.cm-2, continues mod, for 5 minutes daily for 2 weeks. The control group just affected by Dexa. Alkaline phosphatase activity assay and semi-quantitative RT-PCR for alkaline phosphatase, Osteocalcin and Osteopontin expression in 1st, 3rd, 5th, 7th, 9th, 11th and 14th days post-stimulation, respectively. Results: Our results showed that alkaline phosphatase activity in LIUS and Dexa group was significantly more than that of Dexa (p≤0.005). According to semi-quantitative RT-PCR data, differentiation genes expression was significantly different in tow groups (p≤0.005). Conclusion: This study strongly suggests that Dexa and LIUS combination in osteogenic differentiation in rMSCs, was a synergistic effect and has a primitive effect on Osteogenic differentiation.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/375
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