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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۸۲-۸۲
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عنوان انگلیسی Ps-50: Effects of Growth Factor and Shear Stress on The Stability of Differentiation of Mesenchymal Stem Cells, Based on The Expression of VE-cadherin Gene
چکیده انگلیسی مقاله Objective: Mesenchymal stem cells have been proposed as a suitable cell source for vascular tissue engineering, because of their high proliferative capacity as well as their ability to differentiate into various phenotypes. Shear stress due to blood flow is one of the most important mechanical signals experienced by endothelial cells and can be used as a tool for inducing endothelial differentiation. The purpose of the present work is to examine the effects of endothelial growth factor (VEGF) and shear stress on the differentiation of mesenchymal stem cells into vascular endothelial cells and the stability of this differentiation. Materials and Methods: Adipose-derived mesenchymal stem cells (AdMSCs), were isolated from human fat tissue, and characterized using flow cytometry. These cells were cultured in DMEM containing 20% FBS and passage 3 cells were used in experiments. AdMSCs were injected into collagen coated-tubular silicone scaffolds and exposed to proper culture conditions. Cells were exposed to VEGF (for 7 days), shear stress (for 24 hours) or both VEGF and shear stress. For studying the stability of differentiation, the mRNA levels of the VE-cadherin gene were quantified using Real-Time PCR, upon completion of each test, as well as on the 5th and 10th days after the test. Results: According to the results, immediately upon the completion of tests as well as on the 5th day after the experiments, the mRNA level of VE-cadherin was higher in the mechanical group compared to the chemical and chemical-mechanical ones. But on the 10th day after the test, the highest expression was observed in the chemical-mechanical group. Conclusion: According to the results, VE-cadherin gene expression was increased over time, in all three experimental groups, but this increase with time was more observable in the chemical-mechanical group. This can be due to the simultaneous effects of chemical and mechanical signals on the expression of endothelial genes in AdMSCs over time, compared to the effects of chemical or mechanical cues, alone.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/376
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