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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۸۳-۸۳
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عنوان انگلیسی Ps-51: Analysis of CX3CR Chemokine Gene Expression Involved in Homing in Primed Adipose Tissue Derived Mesenchymal Stem Cells with A Histone Deacethylase Inhibitor
چکیده انگلیسی مقاله Objective: Human adipose tissue-derived mesenchymal stem cells (hAdMSCs) have the potential to restore the function of damaged tissues and, providing promising applications of tissue regenerative medicine strategies. CX3CR gene expression should increase the migratory potential of mesenchymal stem cells. However, the migratory efficiency of mesenchymal stem cells after transplantation becomes limited. To promote the process, treating these cells with valproic acid (VPA) as a histon deacetylase inhibitor can be helpful. Materials and Methods: In this study, we isolated MSCs from adipose tissue. Next, we studied their characteristic functions. MSCs were accordingly isolated from heterogeneous cell populations. Then, 4 passages in culture were primed with VPA 5 mM for 24, 48 and 72hours. Detailed gene expression profiles were investigated using an RNA extraction, and subsequently a reverse transcription polymerase chain reaction (RTPCR) analysis for cDNA synthesis. PCR was used to detect gene expression in MSCs treated and non-treated with VPA. Results: The result showed that treatment with VPA did not promote CX3CR expression in human Adipose derived mesenchymal stem cell. This indicate that VPA treatment could not stimulate migration and homing of mesenchymal stem cells. Conclusion: However, using another concentration and/or different intervals might lead to an increased potential of these cells for cell-based therapies. Furthermore, these cells can be primed in vitro to enhance their in vivo recruitment for tissue repair.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/377
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