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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۸۴-۸۴
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عنوان انگلیسی Ps-52: Analysis of CXCR4 Gene Expression in Primed Adipose Tissue Derived Mesenchymal Stem Cells with A Histone Deacethylase Inhibitor
چکیده انگلیسی مقاله Objective: The migration and homing of mesenchymal stem cells to injured tissues is important for the correction of conditions such as neurodegenerative diseases. Human adipose-derived mesenchymal stem cells (hAdMSCs) have been used for transplantation and cell-based therapies. The multi-potential differentiation of these cells make them excellent options for future tissue engineering and repair. Valproic acid (VPA) is also known to inhibit histone deacetylases (HDACs) and cause diverse effects on mesenchymal stem cells. This study investigated whether treatment of MSCs with VPA would enhance the expression of CXC4 and cell migration. In this study, we isolated MSCs from adipose tissue. Next, we studied their characteristic functions. Materials and Methods: MSCs were isolated from heterogeneous cell populations. After 4 passages in culture, the medium was primed with VPA 5 mM for 24, 48 and 72hours. Detailed gene expression profiles were investigated using a RNA extraction and subsequently a reverse transcription polymerase chain reaction (RTPCR) analysis for cDNA synthesis. PCR was used to detect gene expression in MSCs treated and non-treated with VPA. The CXCR4 expression was observed in the VPA-treated group and control group at mRNA and protein levels. Results: This result demonstrated that treatment with VPA greatly promoted CXCR4 expression in hAdMSCs. We also found that VPA significantly increased CXCR4 expression level in long-term treatments (72 hours). CXCR4 gene expression markedly increased in the VPA-treated groups compared to the control group. Conclusion: These results showed that VPA could considerably improve the migratory potential of human adipose-derived mesenchymal stem cells. It was also found that VPA stimulated MSC migration and homing. Thus, these cells have the potential to be used for cell-based therapies.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/378
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