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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۸۸-۸۸
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عنوان انگلیسی Ps-56: Development of Biocompatible Microfluidic System for Neural Differentiation of Induced Pluripotent Somatic Stem Cells
چکیده انگلیسی مقاله Objective: Controlling cellular orientation, proliferation and differentiation is useful in designing organ replacements and directing tissue regeneration. Materials and Methods: In the present study, we focused on methods to produce microgrooves on biodegradable polymer surfaces, and the influences of physical guidance cues on directing alignment and spatially controlling the in vitro neural differentiation of human Induced pluripotent stem cells (hiPSCs) were studied. Scanning electron microscopy (SEM) imaging, quantitative real time RT-PCR (qPCR) and immunocytochemistry were used to analyze differentiated cells and their expression of nuronal-specific genes. Results: Our observations confirmed the differentiation of hiPSCs to neuronal cells on microfluidic device and suggested their potential application in central nerve system regeneration.significant changes were observed on the neural gene expression with respect to the orientation. Conclusion: According to our results we may conclude that the microfluidic system reported herein could be used as a potential cell carrier for neuronal tissue engineering and a combination of these systems and hiPSCs may have potential application in neuronal regenerative therapy.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/382
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