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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۹۰-۹۰
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عنوان انگلیسی Ps-58: Study of Differentiation Potency of Several Mouse iPSC Clones, Generated by Doxycycline Inducible Lentiviral Transduction System, into Definitive Endoderm Using IDE1 Molecule
چکیده انگلیسی مقاله Objective: Regarding the pluripotency of induced pluripotent stem cells (iPSCs), i.e. no immunological rejection and no ethical issues, iPSCs are considered as an unlimited cell source for potential cell-replacement therapy. Definitive endoderm formation is one of the important steps in the development of organs like pancreas and liver. Because efficiently derived definitive endoderm would potentially serve as an important source for liver and pancreas cell therapy, introducing more efficient protocols for producing definitive endoderm is therefore of great importance. Materials and Methods: In this study, the differentiation potency of three different iPSC clones toward definitive endoderm were assessed. The small molecule IDE1, introduced in 2009 by Melton et al. for definitive endoderm differentiation, was used in this process. Definitive endoderm markers were confirmed using realtime PCR and ICC. Results: The Result of this study showed an increase in the expression level of the endoderm markers SOX17 and GSC and a decrease in the expression level of the ectoderm marker SOX1 after treatment with IDE1. Conclusion: This study indicates that IDE1 promotes definitive endoderm formation more efficiently in comparison with the untreated groups. Among the iPSC clones studied, one clone showed more similarity to the embryonic stem cell (ESC) control in differentiation capability which may be due to the genetic backgrounds and its more similarity to ESC control.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/384
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