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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۹۱-۹۱
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عنوان انگلیسی Ps-59: Chemotactic and Invasive Role of Galectin-3 in Ovarian Cancer
چکیده انگلیسی مقاله Objective: Galectin-3 (Gal-3) binds -galactosides through carbohydrate recognition domain, involving in various processes such as cell proliferation, apoptosis, cell adhesion, invasion, and metastasis. However its role in ovarian cancer remains unknown. The present study sought to determine Gal-3 role in invasion and migration of human ovarian cancer cell line SKOV-3. Materials and Methods: SKOV-3 cells were seeded on the top of transwell inserts (25 × 104 Cells/ well) in the serum free media and lower compartment contained media with 10% FBS. Recombinant human Gal-3 (rh- Gal-3) at 3.3 μM was either added in the upper compartment or in lower compartment alone without serum. In parallel, 0.1% PectaSol-C modified citrus pectin (Pect- MCP) (a competitive inhibitor of Gal-3) was added to the upper compartment in combination with rhGal-3. Invasion assay was performed with matrigel transwells. Migration and invasion were assessed after 5 hours or 20 hours, respectively. Results: There was 2.85-fold increased invasion (p< 0.001) whereas, migration was decreased by 53% (p< 0.01) in the presence of rhGal-3 compared to untreated cells. Moreover, Pect-MCP in combination with rhGal-3 abrogated invasive effect of rhGal-3. However combination of Pect-MCP and rhGal-3 did not change inhibitory effect of rhGal-3 on cell migration. Interestingly, rhGal-3 in the lower compartment increased cell migration by 7-fold. Conclusion: This study for the first time demonstrates Gal-3 involvement in ovarian cancer invasion and migration. Moreover, our data showed that Gal-3 may be involved in chemotaxis. Thus, further investigation on the role of Gal-3 may warrant its use as a potential therapeutic tool in ovarian cancer.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/385
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