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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۹۳-۹۳
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عنوان انگلیسی Ps-61: Isolation, Expansion, and Differentiation of Human Fetus Hippocampus Neural Stem Cells
چکیده انگلیسی مقاله Objective: Neural stem cells (NSCs) are the only cells that are capable to regenerate the central nervous system (CNS) except embryonic stem cells. These cells are achievable from different zones in CNS such as sub-ventricular zone, hippocampus, epandymal cells, etc. Neural stem cells have the ability to differentiate to neuron, oligodendrocyte and astrocyte. Also, NSCs create a new approach to treat some neurodegenerative diseases such as Parkinson, Alzheimer and the other CNS disease including brain stroke and spinal cord injury. In this study we have decided to isolate, expand and differentiate human fetus hippocampus neural stem cells to three neural lineages. Materials and Methods: Tissue samples were dissected from hippocampus of legal aborted 20 week human fetus. Each sample was washed 3 times with cold PBS then the trypsin was added and incubates for 5 minutes. To inactive the trypsin, trypsin inhibitor was added to the sample. The isolated cells were cultured in neurobasal media containing B27, bFGF 10 μg/ml and EGF 20 μg/ml. To differentiate the NSCs into three neural lineage cells fetal bovine serum (5%) added to the media. Results: The isolated cells could form some spheres after 5 days called neurospheres and these cells express Nestin. Differentiated cells were fixed and there were some cells expressed β-tubulin (neuron marker), some of them GFAP (astrocyte marker) and a few of them O4 (oligodendrocyte marker). Conclusion: Our study shows that there are some neural stem cells in hippocampus of human fetus which are able to provide three different CNS cells and it could be a good choice for treating CNS disease.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/387
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