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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۹۴-۹۴
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عنوان انگلیسی Ps-62: Spinal Cord Is An Appropriate Source of Neural Stem Cells
چکیده انگلیسی مقاله Objective: In the future a reliable approach for management of neurodegenerative diseases will be cell therapy and using the neural stem cells. One of these diseases is spinal cord injury that caused by trauma in adult male and is one of the most important cause of disability. Neural stem cells isolated from same niches could make better connection and signaling than the cells from other sources. Here, in this study we isolate neural stem cells from adult human spinal cord and human fetus spinal cord. Materials and Methods: For isolating neural stem cells from human adult and fetus spinal cord, the samples were collected from aborted fetus with gestational age 20 week (for human fetus sample) and traumatize spinal cord (for adult samples). after washing with phosphate buffer saline (PBS) containing 15% pen/ strep, the trypsin was added for 5 minutes. Then trypsin inhibitor was added to samples and the suspension passed through cell strainer 70μm.the samples centrifuged for 5 minutes with 110g.the isolated cells were cultured in DMEM/F12 containing 2% B27, 1% N2, 1% pen/strep, 20 ng/ml Epidermal growth factor (EGF) and 10 ng/ml basic fibroblast growth factor (bFGF).For differentiation of these cells to neurons, oligodendrocytes and astrocytes the growth factor were removed and 5% fetal bovine serum was added to their media. Results: After 1 week the isolated cells made some sphere, the sphere forming frequency was stable till passage 10. Also,the isolated cells expressed Nestin and CD133 (markers for neural stem cells). Differentiated cells express β-tubulin and MAP2 (markers for neuron), O4 (oligodendrocyte marker) and GFAP (astrocyte marker). Conclusion: Both human adult and fetus spinal cord are appropriate sources for isolating neural stem cells and using them for spinal cord injury cell therapy; the isolated cells are capable to differentiate to three neural lineage cells (neurons, oligodendrocytes and astrocytes).
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/388
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