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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۹۵-۹۵
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عنوان انگلیسی Ps-63: Differentiation of Wharton’s Jelly Mesenchymal Stem Cells to Neuron and Motor Neuron in Alginate Scaffold
چکیده انگلیسی مقاله Objective: Microenvironment affects the cells’ behavior in different cell types including Mesenchymal Stem Cells (MSCs) by its mechanical properties. The elasticity of the micro-environment in three dimensional (3D) scaffold change the viability, proliferation and differentiation of MSCs. Alginate is a poly saccharine that could be used as a 3D scaffold and its elasticity changes with different concentrations. MSCs could be differentiated to neuron in alginate scaffold and this differentiation would be more in some concentrations than the others. Our goals in this study were to identify the differentiation of MSCs into neurons and motor neurons in the best concentrations for MSCs’ viability and proliferation according to our previous study. Materials and Methods: Whartons’Jelly Mesenchymal stem cells were obtained from fresh umbilical cord after the blood vessels removed and addition of collagenas. The isolated MSCs were cultured in DMEM containing 10% fetal bovine serum and 1% pen/strep. MSCs were positive for CD44. CD105, CD106, and CD90 and negative for CD34 and CD45.Their multipotency was confirmed by differentiating to adipocyte and osteocyte. For neural differentiation the MSCs cultured in 0.25% alginate and 50mM CaCl2 (the optimum concentration for MSCs proliferation and viability) and the retinoic acid 10 μM and basic fibroblast growth factor (bFGF) 20 ng/ml were added to their media. After 1 week, the immunofluorescence staining was performed on the cultured cells in alginate scaffold for β-tubulin (a marker for neuron) and CD271 (motor neuron marker). Results: The expression of β-tubulin and CD271 are more in this 3D alginate scaffold concentration than 2D culture (P value≤0.05). Conclusion: The physical profile of alginate scaffold and its elasticity induces more MSCs to differentiate to neurons and motor neurons. This study indicates that there are some modalities such as the pressure effects of the MSCs’ surrounding area caused by 3D scaffold could affect neural and motor neuron differentiation from Whartons’ Jelly mesenchymal stem cells
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/389
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