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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۹۷-۹۷
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عنوان انگلیسی Ps-65: Mesenchymal Stem Cells into Germ Cells
چکیده انگلیسی مقاله Objective: Due to problems regarding use of embryonic stem cells (ESCs), many novel sources along with various cultures have been investigated hoping to find a proper alternative. As the objective of the current study, we present the efficacy of a combined medium, retinoic acid together with BMP4, on differentiation process of bone marrow derived mesenchymal stem (BMDMSC) into germ cells. Materials and Methods: To find differentiation capability, after providing purified BMDMSCs, differentiation to osteoblast and adipocyte was confirmed by using appropriate culture medium. To confirm mesenchymal lineage production superficial markers (expression of CD90 and CD73 and non-expression of CD45 and VEGFR2) were investigated by flowcytometry. Then the cells were differentiated to germ cells in mediums containing Retinoic Acid for 7 days and then BMP4 for 4 days. To evaluate germ cells characteristic markers (Mvh, Dazl) flowcytometry and immunoflourescence were used. Results: After 4 passage BMDMSCs were fibroblastlike cells.The cells were able to differentiate into osteoblast and adipocyte cells. Presentation of stem cell superficial markers and absence of endothelial and blood cell markers were confirmative for mesenchymal origination of these cells. This fact was representative for multipotential entity of the examined cells. The flowcytometry and immunoflourescence results showed expression of germ cells characteristic markers. Conclusion: By this study, it was found that germ cell markers were expressed in BMDMSCs after adding exogenous Retinoic Acid into culture medium.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/391
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