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Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۰۱-۱۰۱
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عنوان انگلیسی Ps-69: Neuronal Class Determination: It Is All about Design and Equilibrium
چکیده انگلیسی مقاله Objective: During differentiation a regular change in cellular biochemistry and function gives rise to specialized cell types. The process of differentiation is generally divided into two stages: specification and determination. At specification stage, the commitment is still capable of being reversed. But determination is a multistep and usually but not always irreversible process. It has been proposed that terminal selector genes define assets of neuron classes without touching overall neuronal identity and are, therefore, determinants of neural stem cells. Another feature of determination is robustness in the neuron regardless of changes in the internal and external environment. Materials and Methods: In this study, we address the components of the odorant receptor cis-regulatory regions required for robust odorant receptor expression in Drosophila by the use of bioinformatics and applying different methods like immunohistochemistry and realtime PCR and strong genetic tools in Drosophila like RNAi gene knockdown. Results: Our results show that short clusters with only a few required DNA binding motifs are sufficient to produce expression specificity. These clusters use structural components as motif overlap, order and proximity to provide the specificity. We also show that terminal selector genes are not only the last determinants of olfactory sensory neurons but also are essential for the robust expression of the olfactory receptors. We further demonstrate that the motif cluster for Or59b does not maintain expression if the metabolism or temperature changes. Robustness is moreover produced by the addition of solitary motifs found in the cis-regulatory region of the odorant receptors and the number of these motifs defines the expression strength and robustness of the odorant receptors. Conclusion: Taken together our results support an additive model of the cis-regulatory region of odorant receptors whereby motif clusters permit expression and the number of solitary motifs provide expression level and robustness of the odorant receptors.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/395
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