این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۶، شماره Suppl ۱، صفحات ۱۰۴-۱۰۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ps-72: Co-Culture with Embryonic Stem Cells Improves Neural Differentiation of Adipose Tissue-Derived Stem Cells
چکیده انگلیسی مقاله Objective: Neural differentiation of embryonic and adult stem cells has been reported previously. Although embryonic stem (ES) cells have an excellent potential for differentiation, their clinical application has been confronted by several problems including immune rejection, tumorigenicity and ethical issues. To overcome these problems, transplantation of autologous adult stem cells can be implemented as a more practical and feasible choice. During recent years, adipose tissue has been identified as an accessible and rich source of stem cells with multipotential differentiation capacity. So far, several research groups have reported neural differentiation of adipose tissue-derived stem cells (ADSCs) in low-serum or serum-free media with a cocktail of neural inducing factors. In the present study, we evaluated the effectiveness of a medium containing a synthetic serum replacement (KOSR) for neural differentiation of mouse ADSCs, and compared this medium with lowserum condition. Moreover, we evaluated neural differentiation of the ADSCs following indirect co-culture with ES cells. Materials and Methods: ADSCs from the inguinal adipose tissue of 8-10-weeks old NMRI mice were isolated using 2 mg/ml collagenase A and were characterized using flow cytometry. At first, neural differentiation of the ADSCs was induced under two different culture conditions, DMEM plus 4% FBS and DMEM plus 15% KOSR, with or without β-ME. Then, thirdpassaged ADSCs were indirectly co-cultured with ES cells, and the expression levels of pluripotency markers, Oct4 and Sox2, mesenchymal stem cell markers, CD and CD, and proliferating cell nuclear antigens (PCNA) were assessed in the co-cultured ADSCs. Moreover, the control and co-cultured ADSCs were differentiated to neuron with or without RA treatment. Results: Our findings showed that medium containing KOSR without any additional factor induces neural differentiation of the ADSCs. Two weeks differentiated ADSCs expressed several neuron-specific markers, and RA treatment improved neural differentiation of the ADSCs. The expression levels of Oct4, Sox2 and PCNA were upregulated in the co-cultured ADSCs. Moreover, coculture with the ES cells significantly improved neural differentiation of the ADSCs. Treatment of the cocultured ADSCs with RA diminished the expression of neural maturation markers. Conclusion: Our findings are indicating that mouse ADSCs are capable of neural development in medium containing KOSR. Moreover, co-culture with the ES cells efficiently improves neural differentiation of the ADSCs. Probably, secretion of cytokines, chemokines, interleukins and some growth factors by ES cells have a positive effect on the late maturation of ADSC-derived neurons. Non-contact co-culture with the ES cells may be used as an efficient strategy to improve differentiation potential of adult stem cells for developmental studies and regenerative medicine.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/398
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات